Screening for Deleterious non-synonymous SNPs in Human CCL21 Gene using in-silico analysis

Rheumatoid arthritis (RA) is a chronic, systematic, and progressive inflammatory disorder, causing severe damage to joints hence increase mortality. The Chemokine (C-C motif) ligand 21 (CCL21), member cytokines family, involved in immuno-inflammatory regulatory processes. Therefore, identifying the important SNPs (single nucleotide polymorphisms) CCL21 gene of key importance evaluate their structural functional significance discover novel therapeutic targets for immune-related diseases, including RA. In this study, we used silico approaches most damaging non-synonymous (nsSNPs), playing significant role protein. primary tools study included PROVEAN, SNPs&GO, SIFT PolyPhen2. Other tools, its stability, Structure effect as well conservation profile, were verified using I-Mutant, MutPred, ConSurf. site post-translational modification also predicted. 3-D modeling proteins was carried out I-TASSER which then visualized Chimera v1.11. Furthermore, gene-gene interactions predicted STRING MANIA. It observed that nsSNPs D30Y (rs753133670), I62N (rs1170851787), R75C (rs759733358), R75S (rs776954599) A83V gene. These might have CCL2 protein’s malfunctioning possibly different autoimmune diseases Our concluded that, correlation with certain disorders, i.e. Crohn’s Disease (CD), RA other immune-associated these could be ones. addition, need studied animal models cell cultures association identify if they use therapy pharmacogenomics.